Everything about Chaotropic Agent totally explained
A
chaotropic agent, also known as
chaotropic reagent and
chaotrope', is a substance which disrupts the three dimensional structure in
macromolecules such as
proteins,
DNA, or
RNA and
denatures them. Chaotropic agents interfere with stabilizing intra-molecular interactions mediated by non-
covalent forces such as
hydrogen bonds,
Van der Waals forces, and
hydrophobic effects.
Often structural features, as detected by means such as
circular dichroism can be titrated in a chaotrope concentration-dependent fashion.
Chaotropic reagents include:
High generic salts can have chaotropic properties, by shielding charges and preventing the stabilization of salt bridges. Hydrogen bonding is stronger in nonpolar media, so salts, which increase the
dipole moment of the
solvent, can also destabilize hydrogen bonding.
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